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KMID : 0380619830150030209
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Korean Journal of Food Science and Technology
1983 Volume.15 No. 3 p.209 ~ p.214
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Comparative Studies on the Assay Methods of Stevia Sweeteners
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김남수/Kim, Nam Soo
서기봉/남영중/민병용/오상룡/Suh, Kee Bong/Nam, Young Jung/Min, Byong Yong/Oh, Sang Lyong
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Abstract
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Analytical methods on Stevia sweeteners are compared for their reproducibilities and recoveries. It is possible to separate stevioside, rebaudioside A, rebaudioside C, and dulcoside A through HFLC analysis. Steviolbioside, in addition to above 4 Stevia sweeteners, is detected through TLC scanner and TLC-FID assays. C.V.s on stevioside and rebaudioside A in HPLC analysis are 1.39 and 4.89%, which shows outstanding reproducibilities of this method. The recoveries of stevioside in HPLC, TLC scanner, and TLC-FID analyses are 97.7 89.4, and 97.3%. The recoveries of rebaudioside A in HPLC, TLC scanner, and TLC-FID assays are 90.8, 90.1, and 75.8%. Total content of Stevia sweeteners in 8 strains tested, ranges from 5 to 17% as dry weight basis.
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